| Class 1A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Jefferson County (Monticello) | Monticello | ||
| Munroe (Quincy) | Havana | ||
| St. John Paul II (Tallahassee) | Tallahassee | ||
| Class 1A - Region R1 - District D2 | |||
| School | City | ||
| Christs Church Academy (Jacksonville) | Jacksonville | ||
| Cornerstone Classical (Jacksonville) | Jacksonville | ||
| St. Johns Country Day (Orange Park) | Orange Park | ||
| Class 1A - Region R1 - District D3 | |||
| School | City | ||
| First Baptist (Palm Coast) | Palm Coast | ||
| Lake Mary Prep | Lake Mary | ||
| Saint Francis (Gainesville) | Gainesville | ||
| Seven Rivers Christian (Lecanto) | Lecanto | ||
| Class 1A - Region R2 - District D4 | |||
| School | City | ||
| Geneva (Casselberry) | Casselberry | ||
| International Community (Winter Park) | Winter Park | ||
| Legacy (Ocoee) | Ocoee | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Atlantic Christian (West Palm Beach) | West Palm Beach | ||
| Berean Christian (West Palm Beach) | West Palm Beach | ||
| Masters Academy (Vero Beach) | Vero Beach | ||
| Pine School (Hobe Sound) | Hobe Sound | ||
| St. Edwards (Vero Beach) | Vero Beach | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Boca Raton Christian | Boca Raton | ||
| Glades Day (Belle Glade) | Belle Glade | ||
| Lake Worth Christian (Boynton Beach) | Boynton Beach | ||
| Class 1A - Region R3 - District D7 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| All Saints' (Winter Haven) | Winter Haven | ||
| Universal Academy (Tampa) | Tampa | ||
| Victory Christian (Lakeland) | Lakeland | ||
| Class 1A - Region R3 - District D8 | |||
| School | City | ||
| Canterbury (St. Petersburg) | St. Petersburg | ||
| Keswick Christian (St. Petersburg) | St. Petersburg | ||
| Lakeside Christian (Clearwater) | Clearwater | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Bradenton Christian | Bradenton | ||
| Sarasota Christian | Sarasota | ||
| St. Stephens (Bradenton) | Bradenton | ||
| Class 1A - Region R4 - District D10 | |||
| School | City | ||
| Ave Maria | Ave Maria | ||
| Seacrest Country Day (Naples) | Naples | ||
| Village School (Naples) | Naples | ||