| Class 1A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Community Christian (Tallahassee) | Tallahassee | ||
| Crossroad Academy (Quincy) | Quincy | ||
| Jefferson County (Monticello) | Monticello | ||
| Munroe (Quincy) | Havana | ||
| Palm Bay Prep (Panama City) | Panama City | ||
| St. John Paul II (Tallahassee) | Tallahassee | ||
| Class 1A - Region R1 - District D2 | |||
| School | City | ||
| Christs Church Academy (Jacksonville) | Jacksonville | ||
| Cornerstone Classical (Jacksonville) | Jacksonville | ||
| Harvest Community (Jacksonville) | Jacksonville | ||
| San Jose Prep (Jacksonville) | Jacksonville | ||
| St. Johns Country Day (Orange Park) | Orange Park | ||
| Class 1A - Region R1 - District D3 | |||
| School | City | ||
| First Academy-Leesburg | Leesburg | ||
| Redeemer Christian (Ocala) | Ocala | ||
| Saint Francis (Gainesville) | Gainesville | ||
| Seven Rivers Christian (Lecanto) | Lecanto | ||
| Class 1A - Region R1 - District D4 | |||
| School | City | ||
| First Baptist (Palm Coast) | Palm Coast | ||
| Holy Cross (Sanford) | Sanford | ||
| Lake Mary Prep | Lake Mary | ||
| Trinity Christian (Deltona) | Deltona | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Altamonte Christian (Altamonte Springs) | Altamonte Springs | ||
| Geneva (Casselberry) | Casselberry | ||
| International Community (Winter Park) | Winter Park | ||
| Orlando Christian Prep | Orlando | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Central Florida Christian (Orlando) | Orlando | ||
| Faith Christian (Orlando) | Orlando | ||
| Legacy (Ocoee) | Ocoee | ||
| Merritt Island Christian | Merritt Island | ||
| Pineapple Cove (West Melbourne) | West Melbourne | ||
| Class 1A - Region R2 - District D7 | |||
| School | City | ||
| Atlantic Christian (West Palm Beach) | West Palm Beach | ||
| Berean Christian (West Palm Beach) | West Palm Beach | ||
| Masters Academy (Vero Beach) | Vero Beach | ||
| Pine School (Hobe Sound) | Hobe Sound | ||
| St. Edwards (Vero Beach) | Vero Beach | ||
| Class 1A - Region R2 - District D8 | |||
| School | City | ||
| Boca Raton Christian | Boca Raton | ||
| Donna Klein Jewish (Boca Raton) | Boca Raton | ||
| Glades Day (Belle Glade) | Belle Glade | ||
| Lake Worth Christian (Boynton Beach) | Boynton Beach | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Academy at the Lakes (Land O'Lakes) | Land O Lakes | ||
| Classical Prep (Shady Hills) | Shady Hills | ||
| Hernando Christian (Brooksville) | Brooksville | ||
| North Tampa Christian (Wesley Chapel) | Wesley Chapel | ||
| Zephyrhills Christian | Zephyrhills | ||
| Class 1A - Region R3 - District D10 | |||
| School | City | ||
| All Saints' (Winter Haven) | Winter Haven | ||
| Bayshore Christian (Tampa) | Tampa | ||
| Foundation Christian (Valrico) | Valrico | ||
| Riverview Academy | Riverview | ||
| Universal Academy (Tampa) | Tampa | ||
| Victory Christian (Lakeland) | Lakeland | ||