| Class 5A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Gulf Breeze | Gulf Breeze | ||
| Milton | Milton | ||
| Niceville | Niceville | ||
| Tate (Cantonment) | Cantonment | ||
| Class 5A - Region R1 - District D2 | |||
| School | City | ||
| Chiles (Tallahassee) | Tallahassee | ||
| Leon (Tallahassee) | Tallahassee | ||
| Lincoln (Tallahassee) | Tallahassee | ||
| Mosley (Lynn Haven) | Lynn Haven | ||
| Class 5A - Region R1 - District D3 | |||
| School | City | ||
| Clay (Green Cove Springs) | Green Cove Springs | ||
| Gainesville | Gainesville | ||
| Middleburg | Middleburg | ||
| Oakleaf (Orange Park) | Orange Park | ||
| Orange Park | Orange Park | ||
| Ridgeview (Orange Park) | Orange Park | ||
| Class 5A - Region R1 - District D4 | |||
| School | City | ||
| Bartram Trail (St. Johns) | St. Johns | ||
| Fletcher (Neptune Beach) | Neptune Beach | ||
| Matanzas (Palm Coast) | Palm Coast | ||
| Nease (Ponte Vedra) | Ponte Vedra | ||
| Ponte Vedra | Ponte Vedra | ||
| Class 5A - Region R2 - District D5 | |||
| School | City | ||
| Deltona | Deltona | ||
| East River (Orlando) | Orlando | ||
| Edgewater (Orlando) | Orlando | ||
| Evans (Orlando) | Orlando | ||
| Lake Howell (Winter Park) | Winter Park | ||
| Lyman (Longwood) | Longwood | ||
| Wekiva (Apopka) | Apopka | ||
| Winter Springs | Winter Springs | ||
| Class 5A - Region R2 - District D6 | |||
| School | City | ||
| Gateway (Kissimmee) | Kissimmee | ||
| Lake Buena Vista (Orlando) | Orlando | ||
| Lake Minneola | Minneola | ||
| Lake Weir (Ocala) | Ocala | ||
| Osceola (Kissimmee) | Kissimmee | ||
| South Lake (Groveland) | Groveland | ||
| South Marion (Ocala) | Ocala | ||
| Class 5A - Region R2 - District D7 | |||
| School | City | ||
| Bayside (Palm Bay) | Palm Bay | ||
| Fort Pierce Westwood | Fort Pierce | ||
| Heritage (Palm Bay) | Palm Bay | ||
| Melbourne | Melbourne | ||
| Sebastian River (Sebastian) | Sebastian | ||
| Viera (Melbourne) | Viera | ||
| Class 5A - Region R2 - District D8 | |||
| School | City | ||
| Auburndale | Auburndale | ||
| Bartow | Bartow | ||
| Kathleen (Lakeland) | Lakeland | ||
| Lake Gibson (Lakeland) | Lakeland | ||
| Lakeland | Lakeland | ||
| Sebring | Sebring | ||
| Class 5A - Region R3 - District D9 | |||
| School | City | ||
| Cypress Creek (Wesley Chapel) | Wesley Chapel | ||
| East Lake (Tarpon Springs) | Tarpon Springs | ||
| Land O Lakes | Land O Lakes | ||
| Mitchell (New Port Richey) | New Port Richey | ||
| Springstead (Spring Hill) | Spring Hill | ||
| Sunlake (Land O'Lakes) | Land O Lakes | ||
| Wesley Chapel | Wesley Chapel | ||
| Class 5A - Region R3 - District D10 | |||
| School | City | ||
| Freedom (Tampa) | Tampa | ||
| Gaither (Tampa) | Tampa | ||
| Leto (Tampa) | Tampa | ||
| Spoto (Riverview) | Riverview | ||
| Tampa Bay Tech | Tampa | ||
| Wharton (Tampa) | Tampa | ||