| Class 3A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Andrew Jackson (Jacksonville) | Jacksonville | ||
| Baker County (Glen St. Mary) | Glen St. Mary | ||
| Baldwin | Baldwin | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Fernandina Beach | Fernandina Beach | ||
| Raines (Jacksonville) | Jacksonville | ||
| West Nassau (Callahan) | Callahan | ||
| Wolfson (Jacksonville) | Jacksonville | ||
| Class 3A - Region R1 - District D4 | |||
| School | City | ||
| Crystal River | Crystal River | ||
| Dunnellon | Dunnellon | ||
| Eastside (Gainesville) | Gainesville | ||
| Keystone Heights | Keystone Heights | ||
| Newberry | Newberry | ||
| Palatka | Palatka | ||
| Santa Fe (Alachua) | Alachua | ||
| Class 3A - Region R2 - District D5 | |||
| School | City | ||
| Eustis | Eustis | ||
| Hernando (Brooksville) | Brooksville | ||
| Montverde Academy | Montverde | ||
| Nature Coast (Brooksville) | Brooksville | ||
| South Sumter (Bushnell) | Bushnell | ||
| Umatilla | Umatilla | ||
| Villages | Middleton | ||
| Wendell Krinn (New Port Richey) | New Port Richey | ||
| Class 3A - Region R2 - District D6 | |||
| School | City | ||
| Atlantic (Port Orange) | Port Orange | ||
| Four Corners (Davenport) | Davenport | ||
| Lake Highland Prep (Orlando) | Orlando | ||
| Liberty (Kissimmee) | Kissimmee | ||
| Nova Lakes (St. Cloud) | St. Cloud | ||
| Class 3A - Region R2 - District D7 | |||
| School | City | ||
| Chamberlain (Tampa) | Tampa | ||
| Discovery (Lake Alfred) | Lake Alfred | ||
| Holy Names (Tampa) | Tampa | ||
| Jefferson (Tampa) | Tampa | ||
| King (Tampa) | Tampa | ||
| McKeel (Lakeland) | Lakeland | ||
| Tampa Catholic | Tampa | ||
| Winthrop (Riverview) | Riverview | ||
| Class 3A - Region R2 - District D8 | |||
| School | City | ||
| Astronaut (Titusville) | Titusville | ||
| Cocoa | Cocoa | ||
| Cocoa Beach | Cocoa Beach | ||
| Eau Gallie (Melbourne) | Melbourne | ||
| Merritt Island | Merritt Island | ||
| Palm Bay (Melbourne) | Melbourne | ||
| Space Coast (Cocoa) | Cocoa | ||
| Titusville | Titusville | ||
| Class 3A - Region R3 - District D9 | |||
| School | City | ||
| Anclote (Holiday) | Holiday | ||
| Boca Ciega (Gulfport) | Gulfport | ||
| Calvary Christian (Clearwater) | Clearwater | ||
| Countryside (Clearwater) | Clearwater | ||
| Dunedin | Dunedin | ||
| Lakewood (St. Petersburg) | St. Petersburg | ||
| Tarpon Springs | Tarpon Springs | ||
| Class 3A - Region R3 - District D10 | |||
| School | City | ||
| Bayshore (Bradenton) | Bradenton | ||
| Bishop Verot (Fort Myers) | Fort Myers | ||
| Cardinal Mooney (Sarasota) | Sarasota | ||
| Cypress Lake (Fort Myers) | Fort Myers | ||
| DeSoto County (Arcadia) | Arcadia | ||
| Hardee (Wauchula) | Wauchula | ||
| Oasis (Cape Coral) | Cape Coral | ||
| Wellen Park (North Port) | Venice | ||
| Class 3A - Region R3 - District D11 | |||
| School | City | ||
| Avon Park | Avon Park | ||
| Indian River Charter (Vero Beach) | Vero Beach | ||
| Lake Placid | Lake Placid | ||
| Lincoln Park Academy (Fort Pierce) | Fort Pierce | ||
| Tradition Preparatory (Port St. Lucie) | Port St. Lucie | ||
| Class 3A - Region R3 - District D12 | |||
| School | City | ||
| American Heritage (Delray Beach) | Delray Beach | ||
| Cardinal Newman (West Palm Beach) | West Palm Beach | ||
| Clewiston | Clewiston | ||
| Dreyfoos (West Palm Beach) | West Palm Beach | ||
| Glades Central (Belle Glade) | Belle Glade | ||
| Kings Academy (West Palm Beach) | West Palm Beach | ||
| Somerset Canyons (Boynton Beach) | Boynton Beach | ||