| Class 2A - Region R1 - District D3 | |||
|---|---|---|---|
| School | City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Interlachen | Interlachen | ||
| P. K. Yonge (Gainesville) | Gainesville | ||
| St. Joseph (St. Augustine) | St. Augustine | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Class 2A - Region R2 - District D4 | |||
| School | City | ||
| Mount Dora Christian | Mount Dora | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||
| Class 2A - Region R2 - District D5 | |||
| School | City | ||
| Holy Trinity (Melbourne) | Melbourne | ||
| John Carroll (Fort Pierce) | Fort Pierce | ||
| Melbourne Central Catholic | Melbourne | ||
| Class 2A - Region R2 - District D6 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| First Academy (Orlando) | Orlando | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Windermere Prep | Windermere | ||
| Class 2A - Region R3 - District D7 | |||
| School | City | ||
| Cambridge Christian School (Tampa) | Tampa | ||
| Lakeland Christian | Lakeland | ||
| Santa Fe Catholic (Lakeland) | Lakeland | ||
| Seffner Christian | Seffner | ||
| Class 2A - Region R3 - District D8 | |||
| School | City | ||
| Angeline Academy (Land O'Lakes) | Land O Lakes | ||
| Berkeley Prep (Tampa) | Tampa | ||
| Bishop McLaughlin (Spring Hill) | Spring Hill | ||
| Carrollwood (Tampa) | Tampa | ||
| Class 2A - Region R3 - District D9 | |||
| School | City | ||
| Admiral Farragut (St. Petersburg) | St. Petersburg | ||
| Clearwater Central Catholic | Clearwater | ||
| Indian Rocks Christian (Largo) | Largo | ||
| Northside Christian (St. Petersburg) | St. Petersburg | ||
| Shorecrest Prep (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Class 2A - Region R4 - District D10 | |||
| School | City | ||
| Babcock (Babcock Ranch) | Babcock Ranch | ||
| Community School (Naples) | Naples | ||
| Evangelical Christian (Fort Myers) | Fort Myers | ||
| Gateway Charter (Fort Myers) | Fort Myers | ||
| Southwest Florida Christian (Fort Myers) | Fort Myers | ||
| St. John Neumann (Naples) | Naples | ||
| Class 2A - Region R4 - District D11 | |||
| School | City | ||
| AIE Charter (Miami Springs) | Miami Springs | ||
| Carrollton (Miami) | Miami | ||
| Chaminade-Madonna (Hollywood) | Hollywood | ||
| Oxbridge Academy (West Palm Beach) | West Palm Beach | ||
| Westminster Academy (Fort Lauderdale) | Fort Lauderdale | ||
| Class 2A - Region R4 - District D12 | |||
| School | City | ||
| Archbishop Carroll (Miami) | Miami | ||
| Florida Christian (Miami) | Miami | ||
| Mater Bay Academy (Cutler Bay) | Cutler Bay | ||
| Palmer Trinity (Miami) | Palmetto Bay | ||
| Somerset Academy Charter South Homestead | Homestead | ||
| Westminster Christian (Miami) | Palmetto Bay | ||