| Class 1A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Baker | Baker | ||
| Central (Milton) | Milton | ||
| Destin | Destin | ||
| Freeport | Freeport | ||
| Jay | Jay | ||
| Northview (Century) | Bratt | ||
| Seacoast Collegiate (Santa Rosa Beach) | Santa Rosa Beach | ||
| South Walton (Santa Rosa Beach) | Santa Rosa Beach | ||
| Walton (DeFuniak Springs) | DeFuniak Springs | ||
| Class 1A - Region R1 - District D2 | |||
| School | City | ||
| Bozeman (Panama City) | Panama City | ||
| Chipley | Chipley | ||
| Graceville | Graceville | ||
| Holmes County (Bonifay) | Bonifay | ||
| Ponce de Leon | Ponce de Leon | ||
| Poplar Springs (Graceville) | Graceville | ||
| Rutherford (Panama City) | Panama City | ||
| Vernon | Vernon | ||
| Class 1A - Region R2 - District D3 | |||
| School | City | ||
| Altha | Altha | ||
| Blountstown | Blountstown | ||
| Franklin County (Eastpoint) | Eastpoint | ||
| Liberty County (Bristol) | Bristol | ||
| North Bay Haven (Panama City) | Panama City | ||
| Port St. Joe | Port St. Joe | ||
| Wewahitchka | Wewahitchka | ||
| Class 1A - Region R2 - District D4 | |||
| School | City | ||
| Gadsden County (Havana) | Havana | ||
| Hamilton County (Jasper) | Jasper | ||
| Maclay (Tallahassee) | Tallahassee | ||
| Madison County (Madison) | Madison | ||
| Marianna | Marianna | ||
| Sneads | Sneads | ||
| Taylor County (Perry) | Perry | ||
| Class 1A - Region R3 - District D5 | |||
| School | City | ||
| Andrew Jackson (Jacksonville) | Jacksonville | ||
| Bolles (Jacksonville) | Jacksonville | ||
| Episcopal (Jacksonville) | Jacksonville | ||
| Fernandina Beach | Fernandina Beach | ||
| Harvest Community (Jacksonville) | Jacksonville | ||
| Providence (Jacksonville) | Jacksonville | ||
| University Christian (Jacksonville) | Jacksonville | ||
| West Nassau (Callahan) | Callahan | ||
| Class 1A - Region R3 - District D6 | |||
| School | City | ||
| Baldwin | Baldwin | ||
| Bishop Snyder (Jacksonville) | Jacksonville | ||
| Bradford (Starke) | Starke | ||
| Eagles View (Jacksonville) | Jacksonville | ||
| St. Johns Country Day (Orange Park) | Orange Park | ||
| Temple Christian (Jacksonville) | Jacksonville | ||
| Union County (Lake Butler) | Lake Butler | ||
| Class 1A - Region R4 - District D7 | |||
| School | City | ||
| Bell | Bell | ||
| Branford | Branford | ||
| Bronson | Bronson | ||
| Cedar Key | Cedar Key | ||
| Chiefland | Chiefland | ||
| Fort White | Fort White | ||
| Newberry | Newberry | ||
| Trenton | Trenton | ||
| Williston | Williston | ||
| Class 1A - Region R4 - District D8 | |||
| School | City | ||
| Crescent City | Crescent City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Florida Deaf and Blind (St. Augustine) | St. Augustine | ||
| Hawthorne | Hawthorne | ||
| Interlachen | Interlachen | ||
| Keystone Heights | Keystone Heights | ||
| Peniel Baptist (Palatka) | Palatka | ||
| Taylor (Pierson) | Pierson | ||
| Trinity Christian (Deltona) | Deltona | ||
| Class 1A - Region R5 - District D9 | |||
| School | City | ||
| Foundation Academy (Winter Garden) | Winter Garden | ||
| Montverde Academy | Montverde | ||
| Mount Dora Christian | Mount Dora | ||
| St. John Lutheran (Ocala) | Ocala | ||
| Trinity Catholic (Ocala) | Ocala | ||
| Umatilla | Umatilla | ||
| Wildwood | Wildwood | ||
| Windermere Prep | Windermere | ||
| Class 1A - Region R5 - District D10 | |||
| School | City | ||
| Cornerstone Charter (Belle Isle) | Belle Isle | ||
| Faith Christian (Orlando) | Orlando | ||
| First Academy (Orlando) | Orlando | ||
| International Community (Winter Park) | Winter Park | ||
| Lake Mary Prep | Lake Mary | ||
| Orangewood Christian (Maitland) | Maitland | ||
| The Masters Academy (Oviedo) | Oviedo | ||
| Trinity Preparatory (Winter Park) | Winter Park | ||