| Class 1A - Region R1 - District D1 | |||
|---|---|---|---|
| School | City | ||
| Baker | Baker | ||
| Blountstown | Blountstown | ||
| Holmes County (Bonifay) | Bonifay | ||
| Northview (Century) | Bratt | ||
| Port St. Joe | Port St. Joe | ||
| Test | Newport Beach | ||
| Class 1A - Region R1 - District D2 | |||
| School | City | ||
| Chipley | Chipley | ||
| FAMU (Tallahassee) | Tallahassee | ||
| Maclay (Tallahassee) | Tallahassee | ||
| Marianna | Marianna | ||
| North Florida Christian (Tallahassee) | Tallahassee | ||
| Class 1A - Region R1 - District D3 | |||
| School | City | ||
| Fort White | Fort White | ||
| Madison County (Madison) | Madison | ||
| Taylor County (Perry) | Perry | ||
| Class 1A - Region R2 - District D4 | |||
| School | City | ||
| Chiefland | Chiefland | ||
| Hawthorne | Hawthorne | ||
| Williston | Williston | ||
| Class 1A - Region R2 - District D5 | |||
| School | City | ||
| Impact Christian (Jacksonville) | Jacksonville | ||
| Trinity Christian (Jacksonville) | Jacksonville | ||
| University Christian (Jacksonville) | Jacksonville | ||
| Class 1A - Region R2 - District D6 | |||
| School | City | ||
| Father Lopez (Daytona Beach) | Daytona Beach | ||
| Melbourne Central Catholic | Melbourne | ||
| Wildwood | Wildwood | ||
| Class 1A - Region R3 - District D7 | |||
| School | City | ||
| Fort Meade | Fort Meade | ||
| Lakeland Christian | Lakeland | ||
| McLaughlin (Lake Wales) | Lake Wales | ||
| Moore Haven | Moore Haven | ||
| Victory Christian (Lakeland) | Lakeland | ||
| Class 1A - Region R3 - District D8 | |||
| School | City | ||
| Carrollwood (Tampa) | Tampa | ||
| Northside Christian (St. Petersburg) | St. Petersburg | ||
| St. Petersburg Catholic | St. Petersburg | ||
| Zephyrhills Christian | Zephyrhills | ||
| Class 1A - Region R3 - District D9 | |||
| School | City | ||
| Community School (Naples) | Naples | ||
| First Baptist (Naples) | Naples | ||
| St. John Neumann (Naples) | Naples | ||
| Class 1A - Region R4 - District D10 | |||
| School | City | ||
| Benjamin (Palm Beach Gardens) | Palm Beach Gardens | ||
| John Carroll (Fort Pierce) | Fort Pierce | ||
| Pahokee | Pahokee | ||
| Posnack (Davie) | Davie | ||
| Somerset Academy Key (Deerfield Beach) | Deerfield Beach | ||
| Somerset Prep (North Lauderdale) | North Lauderdale | ||